|
On-Chip Living-Cell Microarrays for Network Biology Host Publication: Bioinformatics - Trends and Methodologies Authors: R. Willaert and H. Sahli Publisher: InTech Publication Year: 2011 Number of Pages: 22 ISBN: 978-953-307-282-1
Abstract: The recently developed field of systems biology creates a new framework for understanding
the molecular basis of physiological or pathophysiological states of cells. Screening
modalities that can be used on single cells are needed to study cellular systems biology. The
recent development of cellular microarrays has provided a method for the complex
molecular analysis of living, single cells (Chen & Davies, 2006). Unlike other highthroughput
systems, such as gene expression profiling microarrays or protein microarrays,
cellular microarrays use a printed pattern of geographically distinct spots to probe living
cells, rather than cell lysates, or other non-viable samples. Among the most powerful tools to
assay gene function on a genome-wide scale in the physiological context of intact living cells
are fluorescence microscopy and related imaging techniques (Pepperkok & Ellenberg, 2006).
To enable these techniques to be applied to functional genomics experiments, fluorescence
microscopy is making the transition to a quantitative and high-throughput technology. External Link.
|
